Journal: Animals : an Open Access Journal from MDPI

Article Title: Multi-Omics Insights into Postnatal Skeletal Muscle Development in Duroc Pigs

doi: 10.3390/ani15182715

Figure Lengend Snippet: Phenotypic assessment of SOL, GAS, and PMM in Duroc pigs. ( A ) Experimental design diagram. ( B ) Phenotypic diagrams of SOL, GAS, LDM, and PMM are presented, with LDM as the control for PMM ( n = 6). ( C ) HE staining of SOL, GAS, and PMM across three developmental stages ( n = 6); Scale bar, 50 μm. ( D ) At 120 days of age, MYH7 antibody immunostaining was performed on SOL, GAS, and PMM ( n = 3). ( E , F ) At 120 days of age, the mRNA expression levels of MYH7 and MYH4 in SOL, GAS, and PMM were detected using qRT-PCR ( n = 3). ( G ) Protein expression levels of MYH7 and MYH1 during the development of the SOL, GAS, and PMM were analyzed by Western Blot ( n = 3).

Article Snippet: Western blot analysis was performed with the following primary antibodies: MYH7 (1:1000, 22280-1-AP, Proteintech Group, Inc., Wuhan, China), MYH1 (1:10000, 67299-1-Ig, Proteintech Group, Inc., Wuhan, China), and Anti-GAPDH (1:10000, 10494-1-AP, Proteintech Group, Inc., Wuhan, China).

Techniques: Control, Staining, Immunostaining, Expressing, Quantitative RT-PCR, Western Blot